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A field experiment was conducted during winter season of 2015-16 at agricultural farm of IFTM University, Lodhipur Rajput, Moradabad (UP), India, to evaluate the effect of NPK, Sulphur and FYM on growth and yield of mustard (Brassica juncea L.) in western Uttar Pradesh. The experiment consisted ten treatment combination was laid out in randomized block design with three replications. The result revealed that the highest growth and yield attributing characters recorded with the application of 75% NPK in combination with 40 kg S and 10 MT FYM ha-1. Highest plant height (174.63 cm), number of branches plant-1 (24.47), dry weight (21.47 g), number of siliquae plant-1 (381.40), 1000-seed weight (5.52 g), seed yield (1541.5 kg ha-1) and stover yield (5161.0 kg ha-1) was recorded the application of 75% NPK in combination with 40 kg S and 10 MT FYM ha-1. Net return (Rs. 33119.4) and B: C ratio (1.04) was significantly differ from control. Oil and protein content was significantly influenced with the application of Sulphur and FYM. Significantly higher oil content was recorded at 75% NPK along with 40 kg S and 10 MT FYM ha-1. Protein content was significantly higher in 75% NPK along with 40 kg S and 10 MT FYM ha-1.