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The main target of sustainable agriculture including organic farming is to use natural compounds such as plant aqueous extracts to elevate plant growth and productivity. The subject of the present study is to determine the plant growth and inflorescences production, some biochemical constituents of shoot and inflorescences and antioxidative activities of essential oil obtained from chamomile plants exogenously sprayed with aqueous extracts of dried roselle calyces, turmeric rhizomes, safflower flowers and red beet roots. A pot experiment was conducted during the two successive seasons of 2016/2017 and 2017/2018 in the open field of Experimental Farm of Agricultural Botany Department, Faculty of Agriculture, Ain Shams university, Qalyubia, Egypt. Transplants of chamomile, 45 days old, were separately sprayed after 15 days from transplanting by the four different aqueous extracts and distilled water was used as a control. Generally, spraying with tested plant aqueous extracts on chamomile plants caused high efficiency in growth promotion, inflorescences and essential oil production. Red beet and safflower extracts gave the highest number of branches and inflorescences per plant. Chlorophyll a, b, carotenoids, reducing sugars and amino acids were increased in chamomile shoots when red beet and safflower extracts were sprayed while flavonoids and phenolic compounds were significantly decreased in comparing with roselle and turmeric extract treatments. Different concentrations of essential oil and inflorescences ethanolic extracts obtained from chamomile plants treated with safflower and red beet extracts showed the highest scavenging activities on DPPH radical and lowest IC50 values. Finally, it could be concluded that application of plant aqueous extracts considered as alternative method to chemical compounds which achieved sustainability of organic farming.